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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9071*  Cite 
Description

Murine adipocytes differentiated from 3T3-L1 cells showing intracellular trafficking of GFP-GLUT4 vesicles. GLUT 4 (Glucose Transporter Type 4) is a glucose transport protein that is responsible for insulin-regulated glucose translocation into the cell. Fluorescence TIRFM Image. Leica TIRFM Objective used: 100X 1.46 NA. Images captured with a Hamamatsu EMCCD camera.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CL:0000450
Cell Line
3T3L1
Cellular Component
insulin-responsive compartment
Biological Context
Biological Process
vesicle-mediated transport
Molecular Function
glucose transmembrane transporter activity
Attribution
Names
Yu Chen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9071
Archival Resource Key (ARK)
ark:/b7295/w9cil9071
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 186px 0.1448µm
Y 320px 0.1451µm
Time 0.2 seconds