Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7916^* Cite

Description

Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, and 47 images at 5 sec intervals.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: MC:BALB/c 3T3 fibroblast
Cellular Component: nuclear chromatin

Biological Context

Molecular Function: mobility of nuclear protein

Attribution

Names: Ghosh, Rajarshi; Woodcock, Chris
Pubmed: 20679481
Pubmed: 11130729

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7916
Archival Resource Key (ARK): ark:/b7295/w9cil7916

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: quantity of GFP-protein
Visualization Methods: Green fluorescent proteins from Anthozoa
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.05µm
Y	512px	0.05µm

Time	5 seconds	50