Primary endothelial cells from transgenic mouse embedded in 3D collagen gels of ~ 100 Pa Young's modulus are exposed to 10 micromolar blebbistatin (myosin II ATPase inhibitor). Phase contrast time lapse movie taken on a Nikon TE300 with a 20x 0.5 NA LWD objective. Images captured with an Orca II with 100 ms exposure. Image size is 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds See Fischer et al for details. http://www.ncbi.nlm.nih.gov/pubmed/19185493
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 520px | 0.1274—— |
Y | 490px | 0.1274nm |