NCBI Organism:Saccharomyces cerevisiae, ; Cell Types:; Cell Components:nucleolus; Biological process:;

Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:54888^* Cite

Description

Part of a collage of images of a single nucleolus from yeast strain NOY1051 which has a fixed rDNA array size of 143 repeats. In total 71 of 120 35S ribosomal RNA genes visualized in the Miller chromatin spread from this cell are transcriptionally active. An overview of this nucleolus photographed at low magnification is published in French et al. (2008) Mol Cell Biol. 28:4576-87, Fig. 3A. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447126/figure/f3/

Technical Details

Miller chromatin spread. For yeast technique see: Osheim YN, French SL, Sikes ML, Beyer AL. Electron microscope visualization of RNA transcription and processing in Saccharomyces cerevisiae by Miller chromatin spreading. Methods Mol Biol. 2009;464:55-69. doi: 10.1007/978-1-60327-461-6_4. PMID: 18951179.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cell Type: Single cell
Cellular Component: nucleolus

Biological Context

Biological Process: Transcription (RNA polymerase I)

Attribution

Names: Sarah French
Link: Pubmed
OTHER: Cells grown at 30 degC in rich medium were harvested in mid-log phase.

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL54888
Archival Resource Key (ARK): ark:/b7295/w9cil54888

Grouping This image is part of a group.

Imaging

Image Mode: transmission electron microscopy (TEM)
Visualization Methods: Uranyl acetate; phosphotungstic acid

Sample Preparation

Methods: Image was captured at a magnification of 6600X on electron microscope film, then enlarged an additional 3.25X when printed onto photographic paper and scanned at 600 dpi.

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2955px	1.97nm
Y	4418px	1.97µm