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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45701*  Cite 
Description

Plate 20585 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun M. Gustafsdottir
Vebjorn Ljosa
Katherine L. Sokolnicki
Joshua A. Bittker
Nicole E. Bodycombe
Mark Anthony Bray
Vlado Dancik
Tom Hasaka
C. Suk Yee Hon
Melissa M. Kemp
Kejie Li
Deepika Walpita
Mathias J. Wawer
Todd R. Golub
Stuart L. Schreiber
Paul A. Clemons
Anne E. Carpenter
Alykhan F. Shamji
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
OTHER
1-(1,3-BENZODIOXOL-5-YLCARBONYL)PIPERIDINE2-[1-(4-PIPERONYL)PIPERAZINYL]BENZOTHIAZOLE3-[2-[4-(2-METHOXYPHENYL)PIPERAZIN-1-YL]PYRIMIDO[5,4-B]INDOLE-2,4-DIONE5,7-DICHLOROKYNURENIC ACID5-[(DIMETHYLAMINO)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL45701
Archival Resource Key (ARK)
ark:/b7295/w9cil45701
Compounds
1-(1,3-BENZODIOXOL-5-YLCARBONYL)PIPERIDINE
2-[1-(4-PIPERONYL)PIPERAZINYL]BENZOTHIAZOLE
3-[2-[4-(2-METHOXYPHENYL)PIPERAZIN-1-YL]PYRIMIDO[5,4-B]INDOLE-2,4-DIONE
5,7-DICHLOROKYNURENIC ACID
5-[(DIMETHYLAMINO)METHYL]-3-(1-METHYL-1H-INDOL-3-YL)-1,2,4-OXADIAZOLE
5-METHOXYTRYPTAMINE
5'-N-Ethylcarboxamidoadenosine (NECA)
6-Furfurylaminopurine
6-NITROQUIPAZINE
8-HYDROXY-DPAT
A-3
Acebutolol hydrochloride
Aceclofenac
Acenocoumarol
Acetohexamide
acetyl (N)-s-farnesyl-l-cysteine
Acetylsalicylsalicylic acid
Aconitine
ACONITINE
AG1478
Alverine citrate salt
Amitryptiline hydrochloride
Amoxapine
Amperozide
Amyleine hydrochloride
ANIRACETAM
ANPIRTOLINE
ascomycin (FK-520)
Astemizole
Atovaquone
Azathioprine
Aztreonam
BAY 11-7082
BAY K-8644
Benoxinate hydrochloride
BENZAMIL
Benzamil hydrochloride
Berberine chloride
Betahistine mesylate
bezafibrate
Blebbistatin
BML-257
BML-259
bongkrekic acid
Bromo-7-nitroindazole [3-Bromo-7-nitroindazole]
Bromperidol
Budesonide
bumetanide
BUSPIRONE
CA-074-Me
calphostin C
calyculin A
camptothecin
Canrenoic acid potassium salt
CAPE
Carbamazepine
Cefotaxime sodium salt
CGP-37157
chelerythrine
Chelidonine (+)
Chlorpheniramine maleate
Chlorzoxazone
CINANSERIN
Ciprofloxacin hydrochloride
Clindamycin hydrochloride
clonidine
CLOPROSTENOL
Clorsulon
Cloxacillin sodium salt
Convolamine hydrochloride
Cotinine (-)
CYANOPINDOLOL
Cyclizine hydrochloride
cycloheximide
cycloheximide-N-ethylethanoate
CYCLOPIAZONIC ACID
cyclosporin A
CYPROHEPTADINE
Cytisine (-)
cytochalasin B
Danazol
DANTROLENE
dexamethasone
Dexamethasone acetate
DIAZOXIDE
dibutyrylcyclic AMP
dibutyrylcyclic GMP
Diflorasone Diacetate
Dihydroergocristine mesylate
Diphemanil methylsulfate
Diphenhydramine hydrochloride
diphenyleneiodonium
Dipivefrin hydrochloride
Disulfiram
Dizocilpine maleate
DL-PDMP
DOCOSAHEXAENOIC ACID (22:6 n-3)
DOCOSATRIENOIC ACID (22:3 n-3)
DRB
E-4031
Ebselen
Eburnamonine (-)
EHNA
EICOSAPENTAENOIC ACID (20:5 n-3)
Ellipticine
Emetine dihydrochloride
epibatidine (+/-)
Ethosuximide
Etofylline
etoposide
Famotidine
FARNESYLTHIOACETIC ACID
FCCP
FLECAINIDE
FLUFENAMIC ACID
FLUNARIZINE
FLUOXETINE
Fluoxetine hydrochloride
FLUPERLAPINE
FLUPROSTENOL
Flurandrenolide
Flutamide
Galanthamine hydrobromide
GAMMA-LINOLENIC ACID (18:3 n-6)
Gliclazide
Glimepiride
GLIPIZIDE
GLYBURIDE
Glycocholic acid
Go6976
GW-9662
H7
H-89
HA-1004
Halcinonide
Harmalol hydrochloride dihydrate
Harmane hydrochloride
Harmine hydrochloride
Homatropine hydrobromide (R,S)
Huperzine A [(-)-Huperzine A]
Hydrastine hydrochloride
Hydrocotarnine hydrobromide
Hydroquinine hydrobromide hydrate
Hydroxyzine dihydrochloride
IAA-94
IBMX
Ipratropium bromide
Iproniazide phosphate
Isoquinoline, 6,7-dimethoxy-1-methyl-1,2, hydrochloride
K252A
KETANSERIN
Khellin
L-701,252
L-744,832
LIDOCAINE
Lidocaine hydrochloride
LINOLEAMIDE
LINOLEIC ACID
LINOLENIC ACID (18:3 n-3)
Lobelanidine hydrochloride
Lobeline alpha (-) hydrochoride
LOPERAMIDE
Loperamide hydrochloride
LY-171883
LY-278,584
LY-294002
LY-83583
Lycorine
manumycin A
MAPP, D-erythro
MAPP, L-erythro
m-CHLOROPHENYLBIGUANIDE
MDL-72222
MDL 72832
MDL 73005EF
MEAD ACID (20:3 n-9)
Mefenamic acid
Mepenzolate bromide
Mesoridazine besylate
METHIOTHEPIN
Methocarbamol
METHOPRENE ACID
METHYSERGIDE
Metolazone
Mianserine hydrochloride
Midecamycin
Minaprine dihydrochloride
mitomycin C
MK 212
ML7
ML9
Molsidomine
Mometasone furoate
Monocrotaline
MY-5445
Mycophenolic acid
Myosmine
N9-Isopropylolomoucine
Nalbuphine hydrochloride
Nalidixic acid sodium salt hydrate
Naloxone hydrochloride
Naltrexone hydrochloride dihydrate
N-ARACHIDONOYLGLYCINE
NICARDIPINE
NIFLUMIC ACID
Nifuroxazide
Nitrofurantoin
N-LINOLEOYLGLYCINE
Nomifensine maleate
Norcyclobenzaprine
Noscapine
Ondansetron
Ornidazole
Oxethazaine
OXYMETAZOLINE
Paclitaxel
Palmatine chloride
Papaverine hydrochloride
Pargyline hydrochloride
Parthenolide
Phenacetin
Phenformin hydrochloride
Pheniramine maleate
Picotamide monohydrate
Picrotoxinin
Pirenperone
Pirenzepine dihydrochloride
PIZOTIFEN
Prednisone
Pregnenolone 16alpha carbonitrile
Probenecid
PROSTAGLANDIN A1
PROSTAGLANDIN A2
PROSTAGLANDIN B2
Protoveratrine A
Pseudopelletierine hydrochloride
Quercetine dihydrate
Racecadotril
RAPAMYCIN
Rauwolscine hydrochloride
Retrorsine
REV-5901
Riluzole hydrochloride
RO 04-6790
Ro 20-1724
Ro 31-8220
Rolipram
RS 23597-190
RS 39604
RS 56812
(S)-(-)-Atenolol
SDZ 205,557
Selegiline hydrochloride
Seneciphylline
S-FARNESYL-L-CYSTEINE
siguazodan
Solanine alpha
Spironolactone
SR 57227A
Sulfamethoxazole
Sulfasalazine
Sulindac
Sulmazole
Suxibuzone
Tamoxifen citrate
Tenoxicam
Terfenadine
Tetracaine hydrochloride
TETRAHYDROCANNABINOL-7-OIC ACID
Thiocolchicoside
Tiabendazole
Ticlopidine hydrochloride
Todralazine hydrochloride
Tolazoline hydrochloride
Tolnaftate
Tranylcypromine hydrochloride
Trequinsin
Trimetazidine dihydrochloride
Trimethobenzamide hydrochloride
Trimethylcolchicinic acid
TROPANYL 3,5-DIMETHYLBENZOATE
TROPISETRON
TTNPB
Tubocurarine chloride pentahydrate (+)
Ursolic acid
Venlafaxine HCl
Verapamyl hydrochloride
Vitexin
WIN 55,212-2
WY-14643
Xylazine
Y-25130
Yohimbine hydrochloride
Yohimbinic acid monohydrate
zaprinast