Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:44153^* Cite

Description

The picture represents a 3D-reconstruction (using Imaris software) of confocal/multiphoton (Leica SP5) images collected through the thickness (100 micrometers) of a live lymph node from a bone-marrow transplanted mouse. Cells marked by five fluorescent proteins: Cerulean (cyan), EGFP (green), Venus (yellow), tdTomato (purple), mCherry (red) appear surrounded by collagen matrix (second harmonic generation-SHG, white) at 4 months post-transplant. See also Malide et al. (2012) Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16. This is one of three grouped images obtained using the same imaging strategy.

Technical Details

Imaging was performed using a Leica TCS SP5-AOBS 5-channel confocal/two photon system equipped with multiline Argon, diode 561 nm, HeNe 594 nm and HeNe 633 nm as well as TiSaph two photon laser. Images were recorded with a 25x 0.95 NA water dipping objective lens, and 5 micrometer z-spacing and processed using Imaris (Bitplane). See also: Malide D, Metais J-Y, and Dunbar CE. (2012). Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: lymph node
Cellular Component: cytoplasm

Biological Context

Biological Process

Attribution

Names: Daniela Malide; Jean-Yves Metais; Cynthia Dunbar
OTHER: National Institutes of Heath, Bethesda, MD

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL44153
Archival Resource Key (ARK): ark:/b7295/w9cil44153

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy; multi-photon microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: compartmentalization of stain or label
Visualization Methods: CeruleanFP; EGFP; VenusFP; tdTomatoFP; mCherryFP; second harmonic generation SHG
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	954px	——
Y	1020px	——