Biological process:cellular component organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:43901^* Cite

Description

Tomograms of specifically labeled connexin 43 (Cx43) gap junctions expressed in HEK293T. Cx43 was genetically tagged with GPF and APEX, an engineered plant peroxidase that reacts with hydrogen peroxide and diaminobenzidine to create a protein specific stain. Sections are cut perpendicular to the membrane plane (cross-section view).

Biological Sources

Cell Line: HEK-293 482R

Biological Context

Biological Process: cellular component organization
Molecular Function: intercellular communication

Attribution

Names: Gina Sosinsky; Alice Ting; Jeff Martell; Mark Ellisman; Thomas Deerinck
Published: Nature Biotechnology
Link: CCDB 83884

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL43901
Archival Resource Key (ARK): ark:/b7295/w9cil43901

Imaging

Image Type: recorded image
Image Mode
Parameters Imaged: electron density

Sample Preparation

Methods: glutaraldehyde fixed tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3076px	——