Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41066^* Cite

Description

Fluorescent micrograph of A549 cells (adenocarcinomic human alveolar basal epithelial cells) showing the nucleus (blue) and mRNAs of TFRC (transferrin receptor) identified by fluorescently labeled oligonucleotides.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: A549
Cellular Component: nucleus; transferrin receptor mRNA

Biological Context

Biological Process

Attribution

Names: Arturo Orjalo, Jr.; Sally Coassin; Hans Johansson
OTHER: Jerry Ruth

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41066
Archival Resource Key (ARK): ark:/b7295/w9cil41066

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific nucleic acid sequence; distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); fluorescently labeled oligonucleotides; quasar 570
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1392px	——
Y	1040px	——