Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41035^* Cite

Description

Gallery of transmission electron micrographs of polysomes (polyribosomes) purified from a rat liver homogenate by sucrose gradient centrifugation, and negatively stained with uranyl acetate. The linear arrangement of ribosomes bound to messenger RNA is clearly seen, and in places, the mRNA is visible (arrows).

Technical Details

Samples were placed on carbon coated collodion films, negatively stained with aqueous uranyl acetate, and examined with a Philips 300 TEM. See also N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323

Biological Sources

NCBI Organism Classification: Rattus rattus
Cell Type: hepatocyte
Cellular Component: polysome; ribosome; messenger RNA

Biological Context

Biological Process

Attribution

Names: Yoshiaki Nonomura; Gunter Blobel; David Sabatini; George E. Palade
Published: N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323
Pubmed: 4106575
Link: George E. Palade EM Slide Collection

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41035
Archival Resource Key (ARK): ark:/b7295/w9cil41035

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: processed data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3140px	——
Y	3012px	——