Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40810^* Cite

Description

Colorized transmission electron micrograph of a platinum replica showing the cytoskeletal organization of stubby dendritic spines in extracted hippocampal neurons after 14 DIV. This imge shows branched actin filaments (cyan) in the spine bases associate with dendritic microtubules (red). Image corresponds to Figure 3b in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40952. The uncolorized image is available as CIL 40804, and the insert (this image) is available as CIL 40810.

Technical Details

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Biological Sources

NCBI Organism Classification: Rattus
Cell Type: hippocampal neuron
Cellular Component: axon; dendrite; dendritic spine; actin cytoskeleton; microtubule cytoskeleton

Biological Context

Biological Process: dendrite morphogenesis

Attribution

Names: Farida Korobova; Tatyana Svitkina
Published: Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4.
Pubmed: 19889835

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40810
Archival Resource Key (ARK): ark:/b7295/w9cil40810

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Processing History: colorized

Sample Preparation

Methods: glutaraldehyde fixed tissue; permeabilized tissue; critical_point dried specimen
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	572px	——
Y	479px	——