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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40670*  Cite 
Description

Time lapse movie prepared from fluorescence microscopy time series of a Drosophila S2 cell stably expressing mCherry-alpha-tubulin and spreading on a ConA-coated substrate in the presence of cyto D. Long processes containing microtubule bundles are formed. Microtubule looping and entry into the bundles appears to contribute to the growth of the processes. This movie is part of a grouped set that includes the corresponding time series stack of images.

Technical Details

Time series of widefield fluorescence images were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.49 objective lens, Nikon perfect Focus system and Cascade II EMCCD camera (Roper Scientific)driven by Metamorph software. See also Fig 3C and Supplemental Movie S8 in A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Line
S2
Cellular Component
microtubule
cytoskeleton
Biological Context
Biological Process
cytoskeleton organization
Attribution
Names
Vladimir Gelfand
Adam Larsen
Published
A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156
Pubmed
20566873
Link
Proceedings, National Academy of Sciences, US...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40670
Archival Resource Key (ARK)
ark:/b7295/w9cil40670
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 862px ——
Y 564px ——
Time 40 seconds 61