Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40377^* Cite

Description

Time lapse movie of LLCPK cells stably expressing GFP-EB1, a microtubule tip binding protein. GFP-EB1 fluorescence is seen in microtubules and centrosomes which appear to act as microtubule nucleation sites. The stack of images used to prepare the movie is included in this group of images.

Technical Details

See: KJ Salaycik et al. 2005. Quantification of microtubule growth and dynamics in wound-edge cells. J Cell Sci 118:4113-4122. http://jcs.biologists.org/content/118/18/4113.long

Biological Sources

NCBI Organism Classification: Sus scrofa domestica
Cell Type: kidney epithelial cell
Cell Line: LLC-PK1
Cellular Component: microtubule; microtubule cytoskeleton

Biological Context

Biological Process: mitosis; microtubule cytoskeleton organization; cell cycle

Attribution

Names: Patricia Wadsworth; U Serder Tulu

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40377
Archival Resource Key (ARK): ark:/b7295/w9cil40377

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	650px	——
Y	515px	——

Time	2 seconds	93