Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40366^* Cite

Description

Time series stack of LLCPK cells stably expressing GFP-EB1, a microtubule tip binding protein. GFP-EB1 fluorescence is seen in microtubules and centrosomes which appear to act as microtubule nucleation sites. A movie prepared from the stack is included in this group of images.

Technical Details

See: KJ Salaycik et al. 2005. Quantification of microtubule growth and dynamics in wound-edge cells. J Cell Sci 118:4113-4122. http://jcs.biologists.org/content/118/18/4113.long

Biological Sources

NCBI Organism Classification: Sus scrofa domestica
Cell Type: kidney epithelial cell
Cell Line: LLC-PK1
Cellular Component: microtubule; microtubule cytoskeleton

Biological Context

Biological Process: mitosis; microtubule cytoskeleton organization

Attribution

Names: Patricia Wadsworth; U Serder Tulu

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40366
Archival Resource Key (ARK): ark:/b7295/w9cil40366

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	650px	——
Y	515px	——

Time	2 seconds	93