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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40359*  Cite 
Description

This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.

Technical Details

Rattus norvegicus oligodendrocytes purified from P2 cortex. Cells were fixed on glass coverslips with 4% PFA and immunostained with Phalloidin-FITC (green), alpha-tubulin primary ab./Rhodamine secondary ab (red), RIP (oligodendrocyte marker) primary ab/Cy5 secondary ab (blue), and DAPI (nuclear marker also in blue). Photographed using Leica DMI4000B microscope and 63x HCX PL APO OIL Objective. Voxel size 0.1um.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
oligodendrocyte
Cellular Component
actin cytoskeleton
microtubule
nucleus
RIP
Biological Context
Biological Process
oligodendrocyte development
Attribution
Names
Tomasz Rusielewicz
Carmen Melendez-Vasquez
OTHER
Hunter College
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40359
Archival Resource Key (ARK)
ark:/b7295/w9cil40359
Grouping This image is part of a group.
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px ——
Y 1040px ——