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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40312^* Cite

Description

Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and the nucleoporin Nup358(red)within the nuclear envelope of HeLa cells. The two proteins show a close overlap with Nup358 slightly more distal to the midplane of the nuclear pore complex. Other images in this group show the ultrastructural effects of mutant ALADIN.

Technical Details

HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Nup358 and observed with a Delta-vision deconvolution system. See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell; cervical carcinoma
Cell Line: HeLa
Cellular Component: nucleus; nuclear envelope; nuclear pore

Biological Context

Biological Process: nucleus organization; nuclear transport; nuclear envelope organization

Attribution

Names: Janet Cronshaw; Michael Matunis
Published: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827
Pubmed: 12730363
Link: Proceedings National Academy of Sciences USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40312
Archival Resource Key (ARK): ark:/b7295/w9cil40312

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: wide-field detection; fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Alexa Fluor 488; Alexa Fluor 594; EGFP
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	699px	——
Y	549px	——