NCBI Organism:Rattus norvegicus; Cell Types:CNS neuron (sensu Vertebrata) Cell Components:dendritic spine, dendrite;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39948^* Cite

Description

Tomographic reconstruction of dendritic spines on a dendrite from a medium spiny neuron from the rat neostriatum. This image is a maximum intensity projection of a segment of dendrite in a 4 um section. The cell was filled by intracellular injection of dye and photoconverted. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

Technical Details

The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -66 to 70° in 2° increments. Magnification, 3000X; accelerating voltage, 3.0 MeV.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: CNS neuron (sensu Vertebrata)
Cellular Component: dendritic spine; dendrite

Attribution

Names: Maryann Martone; Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Link: CCDB 12

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39948
Archival Resource Key (ARK): ark:/b7295/w9cil39948

Imaging

Image Type: recorded image
Image Mode: FBbi:00000622; detection of electrons; illumination by electrons
Parameters Imaged: electron density
Visualization Methods: intracellular filling
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	287px	——
Y	623px	——