Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39751^* Cite

Description

Astrocyte cell from live slices taken from the cortex layer 2/3 of an 8 month old GFAP mouse. In this mouse the enhanced green fluorescent protein (EGFP) under the control of the human glial fibrillary acidic protein (GFAP) promoter (line TgN(GFAP-EGFP)GFEC-FKi). In the image, you can clearly see cell the body, astrocyte cloud and astrocyte projection on vascular tube crossing astrocyte cloud (left and up from cell body). This image is the projection of 30 individual z slices. Single z slices from the stack are available as CIL 39752 and 39753.

Technical Details

For details on specimen preparation, please see: Palygin O, Lalo U, Verkhratsky A, Pankratov Y. Cell Calcium. 2010 Oct;48(4):225-31 and Lalo U, Palygin O, North RA, Verkhratsky A, Pankratov Y. Aging Cell. 2011 Jun;10(3):392-402. doi: 10.1111/j.1474-9726.2011.00682.x. Epub 2011 Mar 22.

Biological Sources

NCBI Organism Classification: mus musculus
Cell Type: astrocyte
Cellular Component: cell projection

Biological Context

Biological Process: cerebellar cortex structural organization

Attribution

Names: Oleg Palygin
OTHER: Department of Physiology The Medical College of Wisconsin

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39751
Archival Resource Key (ARK): ark:/b7295/w9cil39751

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: two-photon laser scanning micrograph
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: projection

Sample Preparation

Methods: brian slices
Relation To Intact Cell: living tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	572px	——
Y	599px	——