Astrocyte cell from live slices taken from the cortex layer 2/3 of an 8 month old GFAP mouse. In this mouse the enhanced green fluorescent protein (EGFP) under the control of the human glial fibrillary acidic protein (GFAP) promoter (line TgN(GFAP-EGFP)GFEC-FKi). In the image, you can clearly see cell the body, astrocyte cloud and astrocyte projection on vascular tube crossing astrocyte cloud (left and up from cell body). This image is the projection of 30 individual z slices. Single z slices from the stack are available as CIL 39752 and 39753.
For details on specimen preparation, please see: Palygin O, Lalo U, Verkhratsky A, Pankratov Y. Cell Calcium. 2010 Oct;48(4):225-31 and Lalo U, Palygin O, North RA, Verkhratsky A, Pankratov Y. Aging Cell. 2011 Jun;10(3):392-402. doi: 10.1111/j.1474-9726.2011.00682.x. Epub 2011 Mar 22.
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X | 572px | —— |
Y | 599px | —— |