TEM image of frozen hydrated soluble chromatin isolated from COS-7 cells and vitrified in 15 mM monovalent ions. The chromatin adopts an irregular zigzag fiber conformation. See Fig 2 in J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178.
Isolated nuclei were prepared from COS-7 African Green Monkey and polynucleosomes released after treatment with micrococcal nuclease. Samples were fixed with 0.1% glutaraldehyde for 4 hr at 4C, placed on holey carbon films, vitrified by plunge freezing, and observed in the frozen hydrated state using a Philips CM10 TEM operated at 60KV. Low dose images were recorded on Kodak SO163 film. See also: J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 637px | —— |
| Y | 500px | —— |
