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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39554*  Cite 
Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged NET1. NET1 is a core subunit of the RENT complex that involves in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure. These phase, gfp images are part of an image group that ranges from CIL:39551-39555. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
NET1 protein
nucleus
RENT complex
nucleolus
Attribution
Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39554
Archival Resource Key (ARK)
ark:/b7295/w9cil39554
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Z 21px 0.5µm
Time 300 microns 61