Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39406^* Cite

Description

11 micrographs of views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 5 food vacuoles and the edges of 2 more. The connection of the stalk with the cell body and the myonemes merging into the spasmoneme that passes into the contractile stalk are seen. The aboral end of the elongated macronucleus now appears. TEM taken on 4/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 2,150X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: cell; food vacuole; macronucleus; contractile stalk

Biological Context

Biological Process: digestive system process; cytoplasm organization; cortical cytoskeleton organization; macronucleus organization; contractile stalk organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39406
Archival Resource Key (ARK): ark:/b7295/w9cil39406

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3635px	2.3nm
Y	4000px	2.3nm