Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39338^* Cite

Description

A view of the AZM with basal bodies in longitudinal section and cilia sectioned lengthwise. Bunches of microtubules reside below the basal bodies and lie close to the cytopharyngeal vesicles. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Biological Sources

NCBI Organism Classification: Euplotes sp.
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Euplotes sp. from Carolina Biological Supply
Cellular Component: cilium; cilium axoneme; microtubule basal body

Biological Context

Biological Process: ciliary or flagellar motility; microtubule basal body organization; cytoplasm organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39338
Archival Resource Key (ARK): ark:/b7295/w9cil39338

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5168px	0.8nm
Y	6157px	0.8nm