Focused ion beam scanning electron microscopy image series of a region of cell-cell contact between two cells in the multilayered region of an FGF2 (fibroblast growth factor 2) induced mammary organoid in 3D Matrigel culture. Individual images are at 17 nm intervals. This image is part of a series CIL:39301-39305.
Resin-embedded samples were trimmed with a thin razor blade to expose the area of interest on both the top and one side of the block. This was then glued to a SEM stub using colloidal silver paint. Milling and imaging of the block was carried out using a FEI Strata 235 Dual Beam FIB (FEI Inc., Hillsboro, OR). FIB milling at 50 pA generated a beam size of approximately 17 nm. 1k by 1k images were collected with a backscatter electron detector at 5 kv.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | —— |
| Y | 512px | —— |
| Z | —— | 17nm |
