Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39168^* Cite

Description

This micrograph shows that the discoidal vesicles meet the cytopharyngeal membrane edge-on where they fuse. Besides the ribbon of 12 microtubules abutting the membrane there is a second small ribbon of 2 or 3 microtubules lying against the cytopharyngeal membrane on the posterior side of each ribbon. This narrow ribbon does not curve away from the membrane but remains close to the membrane and its termination marks the boundary line between the cytopharynx and the nascent vacuole membrane. TEM taken on 7/27/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Published in J. Cell Biol. 63:904-922, 1974. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus; coated pit; vesicle membrane

Biological Context

Biological Process: digestion; digestive system process

Attribution

Names: Richard Allen (University of Hawaii)
Published: J Cell Biol. 1974 Dec;63(3):904-22.
Published: 4373478

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39168
Archival Resource Key (ARK): ark:/b7295/w9cil39168

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4524px	1.2nm
Y	5063px	1.2nm