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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39126^* Cite

Description

Thin section of a chloroplast of Arabidopsis thaliana lacking the FZL protein, a member of the dynamin superfamily of proteins. FZL is related to FZO, a protein that mediates fusion between mitochondrial outer membrages in animals and fungi. The characteristic distribution of thylakoids membranes in stacked grana (black arrow) and unstacked stromal regions (white arrows) was disorganized with grana lamellae less uniform in length and stacked in a staggered fashion. Asterisks denote vesicle-like structures that occur in the absence of FZL. The other image in this group (CIL 39125) shows the wildtype structure.

Technical Details

3-week old leaf tissue from Arabidopsis thaliana (eco-type Columbia - Col-0) was fixed, embedded in resin, and 70 nm serial sections cut, stained, and observed by transmission EM. See Fig 2 in H Gao et al, 2006. Proc Natl Acad Sci USA 103:6759-6764.

Biological Sources

NCBI Organism Classification: Arabidopsis thaliana
Cell Type: leaf
Cellular Component: chloroplast; plastid thylakoid; granum

Biological Context

Biological Process: C4 photosynthesis; organelle organization

Attribution

Names: Hongbo Gao; Tammy L Sage; Katherine W Osteryoung
Published: H Gao, TL Sage, KW Osteryoung 2006 FZL, an FZO-like protein in plants is a determinant of thylakoid and chloroplast morphology. Proc Natl Acad Sci USA 103:6759-6764
Pubmed: 21646186
Link: Proceedings National Academy of Science USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39126
Archival Resource Key (ARK): ark:/b7295/w9cil39126

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	250px	——
X	184px	——