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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38714^* Cite

Description

Isolated chloroplas tmembranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Under the isolation conditions used, some stacked thylakoids are formed in vitro (S) in this ac-31 mutant. Also seen are starch grains (St) and a pyrenoid (P). See Fig 18 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM.

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Line: ac-5; ac-31
Cellular Component: chloroplast; plastid; plastid thylakoid membrane

Biological Context

Biological Process: photosynthesis; organelle organization

Attribution

Names: Ursula W. Goodenough; L. Andrew Staehelin
Published: UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed: 4396088
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38714
Archival Resource Key (ARK): ark:/b7295/w9cil38714

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide; uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1494px	——
Y	1140px	——