Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green). This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated. The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13.