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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38598*  Cite 
Description

Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green). This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated. The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
foreskin fibroblast
Cellular Component
focal adhesion
actin cytoskeleton
alpha-actinin
vinculin
Biological Context
Biological Process
molecular organization of focal adhesion components
focal adhesion assembly
Attribution
Names
Hari Shroff
Catherine G Galbraith
James A. Galbraith
Helen White
Jennifer Gillette
Scott Olenych
Michael W. Davidson
Eric Betzig
Published
Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Pubmed
18077327
Link
article
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38598
Archival Resource Key (ARK)
ark:/b7295/w9cil38598
Grouping This image is part of a group.
Imaging
Image Type
portrayed image
Image Mode
PALM (photoactivation localization microscopy)
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Dronpa
tdEos
Processing History
probability density map
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 5787px ——
Y 4865px ——