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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37933^* Cite

Description

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.

Technical Details

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.

Biological Sources

NCBI Organism Classification: Caenorhabditis elegans
Cell Type: early embryonic cell
Cellular Component: cell cortex

Biological Context

Biological Process: embryo development ending in birth or egg hatching; establishment or maintenance of polarity of embryonic epithelium; anterior/posterior axis specification, embryo

Attribution

Names: Nathan W. Goehring; Carsten Hoege; Stephan W. Grill; Anthony A. Hyman
Published: J Cell Biol. 2011. 193(3): 583-594.
Pubmed: 21518794
Link: http://jcb-dataviewer.rupress.org/jcb/browse/...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL37933
Archival Resource Key (ARK): ark:/b7295/w9cil37933

Grouping This image is part of a group.

Imaging

Image Type: recorded image; time lapse microscopy
Image Mode: widefield illumination; fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization Methods: mCherryFP; EGFP; Hoechst
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	382px	0.205µm
Y	360px	0.205µm

Time	30 seconds	50