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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37195*  Cite 
Description

Transmission electron micrograph of a negatively stained mitochondria from the fungi Neurospora. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

References: Palade, G.E. 1952. J. Exp. Med. 95:285-298. Palade, G.E. 1952. Anat. Rec. 114:427-451. Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X64,000.

Biological Sources
NCBI Organism Classification
Neurospora
Cellular Component
mitochondrion
Attribution
Names
W. Stoeckenius
Link
George E. Palade EM slide collection
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37195
Archival Resource Key (ARK)
ark:/b7295/w9cil37195
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
negative staining
Sample Preparation
Methods
osmium tetroxide fixed tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 5231px ——