The first image in a z-series of a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The red channel is the diffraction limited image and the green channel is the SIM image. The maximum projection of both z-series are available as CIL 36147 Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.
Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1904px | 0.0397µm |
| Y | 1900px | 0.0397µm |
