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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36764*  Cite 
Description

Kinetodesmal fibers arise from the proximal ends of the basal bodies and curve upward and to the cell’s right of the kinety. Together with kds arising from more posterior basal bodies they form a stack of overlapping fibers in the longitudinal ridge. A ribbon of microtubules also arises from the proximal end of the basal body and curves posteriorly passing to the right of the kinety. The plasma membrane and outer alveolar membrane were fragmented in this cell. TEM taken on 11/2/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.2µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38900) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cortical microtubule cytoskeleton
cortical fiber
kinetodesmal fiber
Biological Context
Biological Process
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36764
Archival Resource Key (ARK)
ark:/b7295/w9cil36764
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3008px ——
Y 3760px ——