Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36441^* Cite

Description

Confocal image of a Xenopus laevis egg imaged at 119 min post fertilization. The embryos are stained for alpha-tubulin (green) and gamma-tubulin (red).

Technical Details

Images collected on a Zeiss LSM 710 confocal with a 10X 0.3 NA objective using 561 and 633 laser lines.

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fertilized egg
Cellular Component: microtubule

Biological Context

Biological Process: mitosis

Attribution

Names: Martin Wuehr; Timothy Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36441
Archival Resource Key (ARK): ark:/b7295/w9cil36441

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2932px	0.4833µm
Y	2932px	0.4833µm