Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36255^* Cite

Description

The 11 micrographs of the group are views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows the edge of last food vacuole in series; contractile vacuole; bacteria-filled chamber; peristome open to the perioral chamber that opens to the cell’s exterior. TEM taken on 4/1/71 By R. Allen with Hitachi HU11A operating at 75kV. Neg. 2,650X. Bar = 2µm. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40571) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: cell; food vacuole; contractile vacuole; oral apparatus

Biological Context

Biological Process: digestive system process; cytoplasm organization; cortical cytoskeleton organization; oral apparatus organization; contractile vacuole organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36255
Archival Resource Key (ARK): ark:/b7295/w9cil36255

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2606px	——
Y	2760px	——