Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36148^* Cite

Description

The first image in this multi-image tiff file is a stimulated emission depletion image (STED) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the increase in spatial resolution in the STED image compared to the diffraction limited image. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

Technical Details

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Leica STED microscope.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: epithelial cell
Cell Line: Schneider S2
Cellular Component: microtubule

Biological Context

Biological Process: microtubule cytoskeleton organization

Attribution

Names: Victor Cazares; Luke Chao; Till Andlauer; James A Galbraith; Catherine G Galbraith011
Link: MBL Neurobiology Course 2011
OTHER: MBL Neurobiology Course 2011

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36148
Archival Resource Key (ARK): ark:/b7295/w9cil36148

Imaging

Image Type: recorded image
Image Mode: stimulated emission depletion (STED)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488
Processing History: maximum projection

Sample Preparation

Methods: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1269px	0.0252µm
Y	1197px	0.0252µm