Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36147^* Cite

Description

The first image in this multi-image tiff file is a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. The z-series are available as CIL 36797. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

Technical Details

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: epithelial cell
Cell Line: Schneider S2
Cellular Component: microtubule

Biological Context

Biological Process: microtubule cytoskeleton organization

Attribution

Names: Victor Cazares; Luke Chao; James A Galbraith; Catherine G Galbraith
Link: MBL Neurobiology Course 2011
OTHER: MBL Neurobiology Course 2011

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36147
Archival Resource Key (ARK): ark:/b7295/w9cil36147

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: structured illumination microscopy (SIM)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488
Processing History: maximum projection

Sample Preparation

Methods: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1054px	0.0397µm
Y	1028px	0.0397µm