Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36023^* Cite

Description

COS7 cells, cotransfected with GFP-PTP1B, APN-EphA3, and TM-BirA, were stimulated with SA Dynabeads. Cell surface EphA3 was labeled on intact cells with IIIA4 α-EphA3 mAb and detected with Alexa Fluor 546–labeled secondary antibodies by confocal microscopy. PTP1B/EphA3 colocalization is apparent around SA Bynabeads.

Biological Sources

NCBI Organism Classification: Chlorocebus aethiops
Cell Type: permanent cell line cell
Cell Line: COS-7

Biological Context

Biological Process: negative regulation of insulin receptor signaling pathway
Molecular Function: protein tyrosine kinase activity

Attribution

Pubmed: PMID: 21135139

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36023
Archival Resource Key (ARK): ark:/b7295/w9cil36023

Grouping This image is part of a group.

Imaging

Image Type: fluorescence microscopy
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Green fluorescent proteins from Aequorea; Alexa Fluor 546

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	——
Y	1024px	——