Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35580^* Cite

Description

Cultured HeLa cells were arrested in metaphase using nocodazole, immunostained for Bub1, Plk1 and CREST, and counter stained with DAPI. Images shown are maximum Z projections of convolved widefield images. Montage shows the effect of RNAi depletion of Bub1 and Plk1.

Technical Details

Metaphase arrested cells were formaldehyde fixed, permeabilized with Triton X-100, immunostained, and z-stack images obtained at 0.2 micron intervals with a Zeiss Axiovert 200M microscope and 63x 1.4 NA objective lens.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell; cervical carcinoma
Cell Line: HeLa
Cellular Component: kinetochore

Biological Context

Biological Process: mitosis

Attribution

Names: Wii Qi; Zhanyun Tang; Hongtao Yu
Published: Qi et al. 2006 Mol Biol Cell 17:3705-3716
Pubmed: 16760428

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35580
Archival Resource Key (ARK): ark:/b7295/w9cil35580

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI)
Processing History: 3D-deconvolution; pseudocolor applied for merge
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1764px	——
Y	1032px	——