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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35432*  Cite 
Description

This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2)and rescued with the coinjection of YFP-TPX2DN, a truncated version of TPX2, which lacks the 118 first amino acids. Figure 7a, fourth row. doi:10.1371/journal.pone.0003338.g007

Technical Details

Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
oocyte
Cellular Component
microtubule
microtubule organizing center part
nuclear chromosome
Attribution
Names
Stephane Brunet
Julien Dumont
Karen W. Lee
Kazuhisha Kinoshita
Pascale Hikal
Oliver J. Gruss
Bernard Maro
Marie-Helene Verlhac
Pubmed
18833336
Link
http://www.plosone.org/article/info:doi%2F10....
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35432
Archival Resource Key (ARK)
ark:/b7295/w9cil35432
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1310px 0.1µm
Y 310px 0.1µm