Living Lilium pollen tube labeled with carboxy-dichlorofluorofluorescein (green) to mark the vacuole and cytochalasin D TMR to mark endoplasmic reticulum (red) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.
Living lily pollen tubes were labeled and then imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green and red signals from a central plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 983px | —— |
| Y | 242px | —— |
