Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35292^* Cite

Description

Living Lilium pollen tube imaged with DIC optics. Shown is the growing tip with the apical region devoid of cytoplasmic organelles. Other images in the group reveal the distributions of cytoskeletal elements and membranous components.

Technical Details

Germinated lily pollen was imaged with DIC optics using a using a Nikon TE300 inverted microscope with a 40x NA1.3 DIC objective lens and recorded with a Quantix Cool Snap HQ CCD camera. See Lovy-Wheeler et al. 2005, Planta 221:95-104; Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321; Lovy-Wheeler et al. 2006 Plant Cell 18:2182-2193; Cardenas et al. 2008 Plant Physiol 146:1611-1621.

Biological Sources

NCBI Organism Classification: Lilium formosanum
Cell Type: pollen tube
Cellular Component: cytoplasm

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Joseph Kunkel; Tobias I Baskin; Peter K Hepler
OTHER: E.G. Allwood; P.J. Hussey; Juan Cardenas

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35292
Archival Resource Key (ARK): ark:/b7295/w9cil35292

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	364px	——
Y	135px	——