Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35287^* Cite

Description

Fixed Lilium pollen tubes were treated with fluorescent phalloidin to label filamentous actin, and confocal images obtained. A strongly labeled actin fringe near the growing tip is present. Other images in the group reveal the distributions of additional cytoskeletal elements and membranous components.

Technical Details

Germinated lily pollen was fixed with paraformaldehyde and glutaraldehye, treated with Alexa-543 phalloidin to label actin, and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image is a projection of several confocal x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources

NCBI Organism Classification: Lilium formosanum
Cell Type: pollen tube
Cellular Component: actin filament

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Kathleen L Wilsen; Tobias I Baskin; Peter K Hepler

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35287
Archival Resource Key (ARK): ark:/b7295/w9cil35287

Grouping This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT)
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: alexa fluor 543
Processing History: projection of slices
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	403px	——
Y	87px	——