Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35286^* Cite

Description

Freeze substituted Lilium pollen tube immunolabeled for filamentous actin and imaged using laser scanning confocal microscopy. Shown is a projection of x-z slices revealing the cortical distribution of the dense sub-apical actin fringe. This image group also contains a projection of x-y slices of this specimen.

Technical Details

Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-actin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-z slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources

NCBI Organism Classification: Lilium formosanum
Cell Type: pollen tube
Cellular Component: actin cytoskeleton

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Kathleen L Wilsen; Tobias I Baskin; Peter K Hepler

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35286
Archival Resource Key (ARK): ark:/b7295/w9cil35286

Grouping This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT)
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: Cy3
Processing History: projection of confocal slices
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: cryofixed tissue; FBbi:00000621
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	532px	——
Y	216px	——