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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35212*  Cite 
Description

Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. A micropipette is positioned near the cells to deliver either a growth factor or control buffer. In this sequence PBS buffer is added and the cells move independently of the addition. Also of note, a cell can be seen dividing in the lower left.

Technical Details

These data were collected as research which led to the published article Cell Physiol Biochem 2010;25:279-292. Microscopy was performed with a 10X planapo phase contrast objective on an Olympus IX 70 microscope with a SensicamHQ cooled CCD camera and a heated stage insert set to 37 degrees C. Cells were grown and serum starved for 48 h in a coverslip bottom MatTek dish. Small amounts of PDGF-AA were continuously ejected from the micropipette to create a gradient in the vicinity of growth-arrested wt MEFs. Cell movement was monitored with time lapse video microscopy, taking images at 30 second intervals. A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure required for the chemoattractant flow. Femptotip II micropipettes were positioned within 1 μm of the cover slip and pressure set at 30 to 45 hPa. Spatial scale is approximate. Imaging performed at the Image Analysis Facility of the Albert Einstein College of Medicine.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
MEF
Cellular Component
ruffle
Biological Context
Biological Process
cell motility
cytokinesis
Molecular Function
receptor signaling protein tyrosine kinase activity
Attribution
Names
Søren T. Christensen
Linda Schneider
Michael Cammer
Published
Cell Physiol Biochem 2010;25:279-292
Pubmed
20110689
Link
Journal Article
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35212
Archival Resource Key (ARK)
ark:/b7295/w9cil35212
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1280px 0.33µm
Y 1024px 0.33µm
Time 30 seconds 306