Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35203^* Cite

Description

This video shows the dynamics of the apical cell surface of individual cells of the enveloping layer in a killifish embryo. The fluorescent actin is seen to polymerize and depolymerize in cyclic waves that extend and retract these surface protrusions. Two neighboring cells from a continuous epithelium are expressing the fluorescent GFP-actin. The expression of this inserted gene is mosaic, with some cells expressing, and others not.

Technical Details

Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation.

Biological Sources

NCBI Organism Classification: Fundulus heteroclitus
Cell Type: epithelial cell
Cellular Component: actin cytoskeleton

Biological Context

Biological Process: cytoskeleton organization

Attribution

Names: Rachel Fink; Pat Wadsworth
Link: Rachel Fink videos
OTHER: Zebrafish -- The Living Laboratory

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35203
Archival Resource Key (ARK): ark:/b7295/w9cil35203

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	324px	——
Y	240px	——