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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35202*  Cite 
Description

This video shows the apical (outside-facing) side of epithelia cells that form confluent tissues where each cell is tightly glued to its neighbors by circumferential junctions like bands of velcro. These cells show that the cell surface is extremely dynamic, blistering and boiling with small surface protrusions, filled with actin. This video also shows these protrusions in parts of three neighboring cells, and the circumferential actin-filled ridges at the cell margins are particularly dramatic. The actin used in these protrusions is the same pool used by deep cells for individual migration, and these enveloping (EVL) cells use the same kinds of biochemical controls for polymerization (rapid growth) and depolymerization (shrinkage) of filaments.

Technical Details

Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation.

Biological Sources
NCBI Organism Classification
Fundulus heteroclitus
Cell Type
epithelial cell
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
cytoskeleton organization
Attribution
Names
Rachel Fink
Pat Wadsworth
Link
Rachael Fink videos
OTHER
Zebrafish -- The Living Laboratory
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35202
Archival Resource Key (ARK)
ark:/b7295/w9cil35202
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 324px ——
Y 240px ——