Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34517^* Cite

Description

Immunolabeling of endogenous splicing factor SC35 (red) and transfected FP-tagged lamin B receptor. Image stack was acquired using a Deltavision deconvolution microscope.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: cervical carcinoma
Cell Line: HeLa
Cellular Component: spliceosomal complex; lamin-b receptor

Biological Context

Biological Process: RNA splicing; RNA metabolic process

Attribution

Names: Paula Bubulya; David Spector

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34517
Archival Resource Key (ARK): ark:/b7295/w9cil34517

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain; distribution of a specific protein
Processing History: 3D-deconvolution
Data Qualifiers: processed data

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	202px	0.107µm
Y	206px	0.107µm