Image shows a z-series immuno-FISH of a mouse ES cell carrying a multi-copy alpha-globin BAC insertion: HP1 gamma immunostaining (blue) is shown relative to BAC FISH signal (red) versus combined lac operator, vector backbone FISH signal (green).
Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. Pixel dimensions are 0.067 microns (xy) and z spacing is 0.2 microns. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 207px | 0.067µm |
| X | 205px | 0.067µm |
| Z | 15px | 0.2µm |
