Licensing

Attribution Share Alike:This image is licensed under a Creative Commons Attribution, Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26596^* Cite

Description

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The U1-70K protein is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). From Fig 4A in Huranova et al. 2010 J Cell Biol 191:75-86.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: cervical epithelium
Cell Line: HeLa
Cellular Component: pre-mRNA; snRNP; spliceosomal complex

Biological Context

Biological Process: RNA splicing; RNA metabolic process

Attribution

Names: M. Huranova; I. Ivani; A. Benda; I. Poser; Y. Brody; M. Hof; Y. Shav-Tal; K.M. Neugebauer; D. Stanek
Published: M Huranova et al. 2010 J Cell Biol 191:75-86

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL26596
Archival Resource Key (ARK): ark:/b7295/w9cil26596

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	889px	——
Y	889px	——