Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26566^* Cite

Description

Image shows a z-series of a mouse ES cell carrying an alpha-globin BAC transgene array (clone 1c) hybridized with probes against the lac operator repeat (green) and the entire alpha-globin BAC sequence (red). Lac operator sequences formed foci which assumed a more interior location within the engineered chromosome subterritory formed by the BAC transgene array. DAPI counterstaining is shown in blue. Pixel sizes (xy) were 0.074 microns and z-focus steps were 0.2 microns.

Technical Details

Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: embryonic stem cell
Cellular Component: heterochromatin; nuclear chromatin; transgene

Biological Context

Biological Process: nucleus organization; chromatin organization

Attribution

Names: Paul Sinclair; Qian Bian; Matt Plutz; Edith Heard; Andrew Belmont
Published: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776
Pubmed: 20819934
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL26566
Archival Resource Key (ARK): ark:/b7295/w9cil26566

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); EGFP
Processing History: constrained iterative deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.074µm
X	512px	0.074µm
Z	60px	0.2µm