Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25846*  Cite 
Description

Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
CHO-K1
Cellular Component
focal adhesion
myosin II complex
Biological Context
Biological Process
focal adhesion disassembly
Attribution
Names
Miguel Vicente-Manzanares
Karen Newell-Litwa
Alexia I. Bachir
Leanna A. Whitmore
Alan Rick Horwitz
Published
J Cell Biol 2011 193:381-396
Pubmed
21482721
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25846
Archival Resource Key (ARK)
ark:/b7295/w9cil25846
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mCherryFP
Processing History
unproc
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 248px ——
Y 258px ——
Time 5 seconds