Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25359^* Cite

Description

Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.

Biological Sources

NCBI Organism Classification: Canis lupus familiaris
Cell Type: epithelial cell
Cell Line: MDCK
Cellular Component: cell

Biological Context

Biological Process

Attribution

Names: M Pentecost; G Otto; JA Theriot; MR Amieva
Published: PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27
Pubmed: 16446782

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL25359
Archival Resource Key (ARK): ark:/b7295/w9cil25359

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: monolayer

Dimensions

Spatial Axis	Image Size	Pixel Size
X	240px	——
Y	186px	——

Time	600 seconds	43